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The
Leader in Biological Sensing Systems is pleased to make the following
materials available for process testing
Cryptosporidium
parvum oocysts from calf feces
Oocysts are highly viable and supplied with certification
as to the percentage of oocysts negative in the DAPI/PI dye permeability
test
Oocysts are not subjected to any caustic chemicals during
purification
Oocysts are available in lots of 1 billion or greater,
depending on the needs of the test system. Should you require
smaller quantities, for diagnostic purposes, we now offer single
dose sizes (< 1 million oocysts)
Oocysts are collected and purified regularly
Sentinel Chambers loaded with 10 million oocysts
Oocysts are in direct contact with the water or wastewater
of interest
Sentinel Chambers allow rapid equilibration with
the test system
The large number of oocysts in
each chamber makes oocyst recovery easier and less expensive and
supplies easily retrievable oocysts for viability or infectivity
testing
Sentinel Chambers and Cryptosporidium
Oocysts
Sentinel Chambers are loaded with 10 million Cryptosporidium
parvum oocysts and water or wastewater taken directly from the
process system to be tested. Fine mesh screens on the ends of
the Sentinel Chamber hold the oocysts within the chamber
and at the same time allow the interior to equilibrate with the
surrounding environment. After the Sentinel Chamber is in
the test environment for the required period, the Sentinel Chamber
is retrieved, and the oocysts are easily recovered to determine
viability or infectivity following disinfection.
One-Step Reduction Assays
The number of oocysts loaded into each Sentinel Chamber
makes recovery for viability determinations easy and quick to
perform. The 10 million oocysts in each chamber allows for the
easy assessment of 5 LOG reductions when the entire contents of
a chamber are used as the inoculum in infectivity assays.
High Oocyst Viability
The percentage of viable oocysts in each shipment is certified
at greater than 90% viability as determined by the DAPI/PI dye
exclusion assay.
Oocysts from Feces
The Cryptosporidium parvum oocysts used have received no
treatment with harmful chemicals and are wild-type oocysts from
the feces of naturally infected calves.

Environmental Exchange
In the Sentinel Chambers containing oocysts, the two screens
have 5 micron pores. Diffusion through the screens occurs quickly
so that the interior of the Sentinel Chamber rapidly equilibrates
with the surrounding environment. Therefore, the effects of any
disinfection treatment on the oocysts in the outside environment
are reflected by the effects on the oocysts within the sentinel.
We also supply chambers made entirely of porous stainless steel.
Currently available in ranging from 1/4" diameter X 1"
long to 1" X 1" cups holding up to 10ml. The oocysts are
exposed to the outside environment while remaining safe inside the
chamber.
Packaging and Shipment:
Each Sentinel Chamber will be loaded with the oocysts within
our facilities in Ithaca, NY. The Sentinel Chambers can be
loaded with oocysts suspended in water supplied by you, in which
case we will supply pre-paid shipping containers and instructions.
When we receive the samples, we will return the Sentinel Chambers
to you filled with oocysts and your water via overnight express.
If there is no special reason to worry about what kind of water
the oocysts are in, delivery can be made within 24 hours.
| Price
List: |
The
Sentinel Chamber loaded with the client-supplied
water or wastewater:
___Sentinel Chamber containing
10 million oocysts
______Orders
of 10 to 100
______Orders
of more than 100 |
$254 each
$226 each
$202 each |
The
Sentinel Chamber loaded with water, sand or digested
biosolid:
___Sentinel Chamber containing
10 million oocysts
______Orders
of 10 or more
We also have empty chambers.
Please contact us via email or by phone/FAX if you are interested
in obtaining empty Sentinel Chambers. |
$205 each
$185 each |
Bulk
sales of oocysts:
We offer bulk sales of oocysts for studies where it is necessary
to spike large systems for viability determinations. These
oocysts are supplied with the same viability documentation
as those in the Sentinel Chambers. Each 1 billion Cryptosporidium
oocysts are $1,800. Smaller quantities are priced as below:
___10
million oocysts
___100 million oocysts
For non-viable oocysts, we offer Gamma irradiated or Formalin
fixed samples at an additional $75. If you have a special
interest in testing fixed organisms, we would be happy to
work with you and your specified application. |
$300
$600 |

For ordering information, click below
or call or email:
Excelsior Sentinel, Inc.
607-330-1284 (fax/tel.)
email: excelsiorsentinel@hotmail.com
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